The value of determining the activity of myeloperoxidase in neutrophilic leukocytes in patients with atherosclerosis

Attaeva Madina Jamalovna, Vasilenko Vladislav Mikhailovich, Gurizheva Madina Valerianovna, Medialiyeva Rimma Hakimovna, Almova Irina Hadzhiismailovna

Objective: to study the activity of myeloperoxidase in neutrophilic leukocytes in patients with atherosclerosis. Materials and methods: 98 patients with atherosclerosis (mean age – 55.8±6.6 years) were examined in dynamics (1-2 days of inpatient treatment, 10-11 days of therapy). They were divided into 3 groups: the first 37 patients with arterial hypertension (average age -56,3±5.9 years), the second - 38 patients with stable angina of II-III functional class (mean age of 53.4±3.6 years) and the third 23 patients with myocardial infarction and stroke (mean age 57.6±7.2 years). 32 healthy people (mean age -52.2±7.1 years) were observed as a comparison group. For cytochemical study of the activity of myeloperoxidase used the method of Sato and Selkija. Quantitative expression of leukocyte MPO activity was carried out according to the Kaplow principle. Research result. In patients with arterial hypertension on the background of atherosclerosis revealed a significant increase in the activity of intraleukocytic enzyme at all times of observation. The highest rates of activity were observed during hospitalization-2332,1 CONV.units On the background of therapy was observed a marked decrease in the activity of the enzyme in neutrophilic leukocytes - 2241,8 CONV.at angina pectoris, a significant increase in the activity of myeloperoxidase in neutrophilic leukocytes was found in all terms of examination - 2371,6 and 2250,9 usl.ed. In the examined patients with acute myocardial infarction and cerebral circulation disorders, a significant increase in the activity of the intraleukocytic enzyme was revealed both in the first days of the disease and by the end of the second week. Conclusion. Myeloperoxidase activity in neutrophils is one of the markers of atherosclerotic activity, promising diagnostic and prognostic indicators for various manifestations of atherosclerosis. The revealed differences in the indicators of myeloperoxidase activity in patients with hypertension, angina pectoris, myocardial infarction and acute cerebrovascular accident can be used as an additional criterion in the diagnosis of these diseases and the effectiveness of treatment.

Keywords:neutrophilic leukocytes, myeloperoxidase, atherosclerosis, arterial hypertension