Effect of Explant Type and Plant Growth Regulators on Callus induction and Accumulation of Phenolic Compounds in Vaccinium corymbosum L. – cv. Blue-Berry in Vitro

Mohamed GmailRayan Aboueldis, Khusnetdinova Landysh Zavdetovna, Timofeeva Olga Arnoldovna

The present study aims to the production of phenolic compounds from callus culture of Vaccinium corymbosum L., the results showed that, callus induction depending on the source of the explant (leaf, root, and stem segments). The optimum composition of the nutrient media were selected for obtaining a high frequency of callus formation. It was shown that the process of callus induction depended on the composition of the nutrient medium and type of explant. The highest frequency of callus formation were established on a nutrient medium containing 1.0 mg/l NAA in combination with 0.5 mg/l Kin. The fresh weight for leaf, root and stem segment explants were recorded (0.91; 0.89 and 0.66 mg/explant). The maximum content of phenolic compounds were recorded in the callus of root-origin.

Keywords:Vaccinium corymbosum L., Highbush Blueberry, callus formation, in vitro culture, explant, callusgenesis induction, WPM medium, phenolic compounds.