Free radical oxidation and antioxidant status in animals under the action of small doses of the herbicide 2,4-dichlorophenoxyacetic acid

Krasikov Sergey Ivanovich, Boev Mikhail Viktorovich, Sharapova Natalia Vasilievna, Karmanova Daria Sergeevna

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-DA) is a widely used compound in agriculture. Given its ability to accumulate in the environment and bioaccumulate in the body, it is relevant to study the characteristics of the body's reactions to the action of subthreshold doses. Experiments lasting 7 weeks were performed on animals that received drinking water containing 2,4-DA at a concentration of 0.015 mg / L, which is 0.5 maximum permissible concentration (MPC). It was shown that under the influence of small doses of 2,4-dichlorophenoxyacetic acid supplied with drinking water, free radical oxidation processes (an increase in the content of diene conjugates and malondialdehyde) were activated and the enzymes of the antioxidant defense system (superoxide dismutase and catalase) were reduced. In addition, there was an increase in the intensity of free-radical oxidation processes in the total fraction of low-density lipoproteins and very low-density lipoproteins isolated from animal serum, and a decrease in antioxidant mechanisms, which manifested itself in the ability to limit the intensity of spontaneous and iron-induced chemiluminescence in the model system of phospholipids.

Keywords:herbicide 2,4-DA, small doses, rats, oxidative stress, chemiluminescence.