Recombinant expresson of the halophilic ß-N-acetylglucosaminidase of archaeon Halomicrobium SP. LC1Hm

Shevchenko M. A., Lisun V. V., Skripskaya V. V., Yakimov M. M.

Halophilic glycosyl hydrolases capable of degrading complex insoluble natural polysaccharides to soluble oligo- and monomers at high salinity are of great interest from the point of view of fundamental and applied science. Thanks to the screening of various highly saline habitats, many halophilic strains producing various extracellular hydrolases have been found. However, the main problem with using these enzymes is the difficulty in obtaining them. In this work, for the first time, achraeal extracellular glycosyl hydrolase of the GH20 family was successfully expressed using the Haloferax volcanii expression system.

Keywords:halophiles, halophilic glycoside hydrolases, cloning, shuttle vector, Haloferax volcanii.