Evaluation of the advanced glycation end products blockers effectiveness with different mechanism on acute lung injury induced by Influenza virus A/H1N1pdm09

Aleksandrov Andrei G., Savateeva-Lyubimova Tatiana N., Zhilinskaya Irina N.

This article evaluates the protective properties of blockers of advanced glycation end products (AGE) aminoguanidine bicarbonate and pyridoxine hydrochloride against virus-associated acute lung injury / acute respiratory distress syndrome (ALI/ARDS). ALI/ARDS model was performed by intranasal inoculation of the influenza A/California/7/09MA virus at a dose of 1 LD50 in mice. The severity of ALI/ARDS was assessed by the level of hemoglobin oxygen saturation and pathomorphological changes in the lungs. AGE’s level was measured in the supernatant of the lung’s homogenate by fluorescence. Both compounds showed significant role in correction of hypoxia and lung’s damage, as well as the accumulation of AGE’s at various stages of ALI/ARDS. At the same time aminoguanidine bicarbonate showed more protective properties than pyridoxine hydrochloride. Obtained results are the reason for study of the mechanisms of suppressing the formation of AGEs and searching for new compounds that prevent their accumulation.

Keywords:advanced glycation end products, acute lung injury / acute respiratory distress syndrome, influenza infection