Modeling and innovative treatment of an infected liver cyst in a surgical experiment

Musaelyan Ara Gagikovich, Polidanov Maxim Andreevich, Kondrashkin Ivan Evgenievich, Takhmezov Alik Eldarovich, Blokhin Igor Sergeevich, Khokhlova Anastasia Vladimirovna, Alipov Artyom Igorevich

Actuality. To clarify diagnostic standards and introduce new minimally invasive methods of surgical intervention and postoperative therapy, it is necessary to develop methods for creating liver cysts and abscesses. Objective. To substantiate an innovative method of modeling an infected liver cyst (ILC) and the effectiveness of the combined use of low-intensity laser radiation (LILI) and metal nanocomplex (MN) in the surgical treatment of ICP. Materials and methods. ILC modeling was carried out in an experiment on 40 laboratory rabbits, which, under the control of ultrasound, a modified double-light catheter with a balloon at the distal end was inserted into one of the lobes of the liver infected with a staphylococcus and Escherichia coli strain. After 6 days, after the formation of the ILC, the cavity was opened and sanitized. The animals were divided into 4 groups (10 in each) and postoperative treatment of ILC was carried out for 10 days. Animals of the first group underwent standard sanation of the cavity and injection of antibiotics; animals of the second group were prescribed local treatment of the LILI cavity; local application of the suspension with MN was carried out to animals of the third group. The animals of the fourth group were injected MN in combination with the use of LILI. Clinical, planimetric, microbiological, morphological and instrumental methods were used to assess the effectiveness of treatment. Results and discussion. It is proved that the use of an infected two-channel catheter with a balloon on the 6th day of the experiment provides the formation of an ILC with the specified characteristics. The isolated use of LILI and MN reduces the time of obliteration of the ILC cavity. The combined use of LILI and MN provides speeded cleaning of the cavity from the pathogen and its obliteration by the 10th day of treatment.

Keywords:infected liver cyst (ILC), metal nanocomplex (MN) low-intensity laser irradiation (LILI), experiment.