Comparative analysis of polymorphism of microsattelete locus in natural populations of taraxacum officionale in the Northern Caucasus

Dzhambetova Petimat Makhmudovna, Bisultanova Zura Isanovna, Dreeva Fatima Robertovna, Reutova Nina Vasilievna, Atsaeva Maret Makhmudovna

Objective: To assess the genetic diversity of T.officionale Wigg.S.L. populations of the Chechen Republic (CR) and the Kabardino-Balkarian Republic (KBR) at different heights above the sea level (n.a.m.) by comparing SSR profiles. Methods: Microsatellite (SSR) genotyping of genomic DNA of 48 T.officionale samples from 10 populations using 12 TKS primers developed for T.Officionale was performed. Results: 40 SSR loci were found, ranging in size from 110 to 1200 bp. A high level of polymorphism of 5 populations of T.officinale from the CR was revealed at an altitude of 800-950 m.n.m. (74.3%) and low at an altitude of 2000-2200 m.n.m. The level of polymorphism of populations of T.officinale CBD decreased from 68.7% to 9.7% with an increase in altitude above sea level. The degree of polymorphism of lowland samples is higher than that of mountain populations. The degree of genetic divergence between T.officionale CR and CBD samples is shown. A low level of polymorphism of T.officionale of the Elbrus region was noted in comparison with plants of the mountainous territories of the Czech Republic. Conclusions: High genetic variability was revealed in lowland Taraxacum populations. All 12 TKS primers used in the course of work can be used for marking T.officionale.

Keywords:genetic variability, Taraxacum officionale, population, microsatellite genotyping, North Caucasus.