Method for molecular genediagnostic of group C/G streptococci

Abashin Ilya Yurievich, Kozyreva Natalia Gennadievna, Gorbatov Alexander Veniaminovich, Avduevskaya Natalia Nikolaevna

In this mini-review we considered the applied method for identifying the emm-gene in streptococci of groups C and G isolated from cattle, based on polymerase chain reaction (PCR), is a fast and accurate method for diagnosing the pathogen. The technique is based on the uniqueness of the nucleotide sequence of the pathogen, which gives a high degree of specificity. The relevance of the developed method is justified by the lack of information about the exact properties of the M-like protein of streptococci encoded by the emm-gene, which is a cellular surface-anchored protein and which presumably causes resistance to phagocytosis. For veterinary medicine, this is a new approach, in particular, when working with group C and G streptococci in cattle.

Keywords:Streptococci, molecular diagnostics, emm-gene, cattle, genediagnostic