ISOLATION OF NATIVE MICROORGANISMS FROM ARCTIC SOILS USING THE ICHIP METHOD

Kadutskii Valerii, Detsura Anna, Evgrafova Svetlana, Shatalov Aleksandr, Sideleva Elizaveta

The problem of uncultivated forms of microorganisms has existed for a long time and is a challenge for modern microbiology, despite significant advances in molecular biology and understanding of many complex biochemical processes. Modern biotechnology is in dire need of identifying and obtaining new highly effective strains of bacteria and archaea capable of producing such useful substances as antibiotics, biodegradable polymers, polyunsaturated fatty acids, psychrotolerant enzymes; Another important task of biotechnology is the use of strains of microorganisms in the bioremediation of polluted environmental objects with various pollutants. Researchers using non-standard methods and cultivation parameters, which are sometimes quite complex, manage to obtain a pure viable culture of any microorganism. It is widely known that microorganisms with unique biochemical characteristics, enzymes, proteins and other beneficial substances often live in extreme conditions, hydrothermal vents, frozen soils and heavily polluted environmental objects. Such microorganisms often require special substrates and growth factors. In this work, we used the method of isolation chips (iChip) to isolate methylotrophic strains from the soils of the permafrost ecosystem, Meduza Bay, near the village of Dikson, Krasnoyarsk Territory. And compared this method with the classical approach. The obtained strains of methylotrophic bacteria were identified by analyzing the primary structure of the 16s rRNA gene. A total of 8 strains were isolated. The morphology of cells and colonies of the obtained cultures was studied, species identification was carried out using the obtained sequences during sequencing of the 16s rRNA gene.

Keywords:Arctic, methylotrophic microorganisms, arctic soils, methanol, isolation chip