COMBINATION OF POLYMORPHIC ALLELES OF THE EDN1 (RS5370) AND NOS3 (RS2070774) GENES ON DMF INDEX INDICES

Bebyakova Natalia Alexandrovna, Levitsky Sergey Nikolayevich, Davydova Nadezhda Gennadyevna, Galieva Alexandra Sergeevna, Zhibort Ekaterina Leonidovna

The article presents the results of the study to establish the relationship between the presence of polymorphic variants of endothelin-1 (EDN1) (rs5370), endothelial nitric oxide synthase (NOS3) (rs2070774) genes and the intensity of the processes of formation and development of dental caries, assessed by the DMF index. In practically healthy young men and girls living in the territory of the European North, a molecular genetic study by PCR with a fluorescent scheme of product detection in real time mode was carried out, levels of vasoactive endothelial factors - nitric oxide (by biochemical method) and endothelin-1 (by immunologic method) were determined, the DMF index (caries + fillings + extracted teeth) and the degree of carious process intensity under the combined influence of polymorphic variants of EDN1 and NOS3 genes were determined. It was found that the presence of rs5370 and rs2070774 in the genotype led to an increase in the frequency of individuals with decompensated stage of dental caries, and therefore single nucleotide substitutions (SNPs) in EDN1 (rs5370) and SNO3 (rs2070774) genes can be considered as markers of changes in resistance to this disease.

Keywords:gene polymorphism, nitric oxide, endothelin-1, dental caries, DMF index.